Research Assistant I

Updated: March 3, 2020
Job ID: 215739
Status: Full-Time
Regular/Temporary: Regular
Location: Silver Spring, MD, United States

Join the HJF Team!

HJF is seeking a Research Assistant I to support the Host Genomics Section within the Military HIV Research Program (MHRP) located at the Walter Reed Army Institute of Research (WRAIR) in Silver Spring, Maryland. HJF provides scientific, technical and programmatic support services to MHRP. 

The incumbent will be responsible for research and/or development in collaboration with others for projects. Provides laboratory and technical support to senior technical and professional staff.

Responsibilities:

  1. Performs molecular biology laboratory procedures/research, including collection and organization of data; makes detailed observations about experiments; performs basic data analysis and interpretation.
  2. Extracts and purifies DNA and RNA; performs automated procedures using liquid handling instrumentation (BioMek NX and Eppendorf) with AmPure and CleanSeq technology; fragments DNA (Covaris ultrasonicator); quantitates (Nanodrop, Agilent, Qubit), aliquots, and maintains genomic DNA and RNA stocks and dilutions.
  3. Sets-up and performs Next Generation Sequencing using protocols specific for whole genome, amplicon, and RNA sequencing on Illumina instruments (MiSeq, NextSeq, NovaSeq).
  4. Amplifies targets from DNA and RNA using PCR; performs standard (ABI 3730xl sequencer) and Next Generation Sequencing; calls alleles using computer-based software packages (e.g. Assign SBT, Omixon Target, GenDX, NGSengine, Mutation Surveyor).  
  5. Amplifies targets from nucleic acid using PCR and clones into plasmid vector systems.
  6. Assists with the preparation of technical reports, summaries, and protocols.
  7. Maintains clear and accurate laboratory records, inventories and logbooks.
  8. Performs data entry for research projects and maintains databases.
  9. Maintains compliance with established laboratory safety and other procedures. 
  10. Provides quality control, trouble-shooting, and maintenance of specialized reagents and equipment.
  11. Maintains a laboratory notebook.
  12. Presents results/data in weekly laboratory meetings.
  13. Maintains a safe and uncluttered work area with appropriate disposal of potentially infectious waste and chemical waste.
  14. Performs other duties as assigned.

Required Knowledge, Skills, and Abilities:  Knowledge of standard and advanced molecular biology laboratory techniques; solid computer literacy; ability to follow general instructions; good communication and analytical skills.

Minimum Education/Training Requirements:  BS in Scientific discipline required.

Minimum Experience:  0 to 2 years of laboratory experience required.

Physical Capabilities:  Long periods of standing and sitting; handling various chemicals.

Work Environment:  Laboratory and computer workstation environments.

Background/Security: Must have lived in the US 3 out of the past 5 years; eligible to pass a NACI background.

Employment with HJF is contingent upon successful completion of a background check, which may include, but is not limited to, contacting your professional references, verification of previous employment, addresses, education, and credentials, a criminal background check, drug screening, and a department of motor vehicle (DMV) check.  

HJF is an equal opportunity and affirmative action employer.  All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status.

Any qualifications to be considered as equivalents, in lieu of stated minimums, require the prior approval of the Chief Human Resources Officer.
 

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